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Microbubbles Remove Listeria monocytogenes from the Surface of Stainless Steel, Cucumber, and Avocado
Pengyu Chen1, Joseph Eifert1, Sunghwan Jung2
1Department of Food Science and Technology, Virginia Tech, Blacksburg, VA 24061, USA.
Abstract:
Fresh produce may be contaminated by bacterial pathogens, including Listeria monocytogenes, during harvesting, packaging, or transporting. A low-intensity cavitation process with air being injected into water was studied to determine the microbubbles' efficiency when detaching L. monocytogenes from stainless steel and the surface of fresh cucumber and avocado. Stainless steel coupons (1″ × 2″), cucumber, and avocado surfaces were inoculated with L. monocytogenes (LCDC strain). After 1, 24 or 48 h, loosely attached cells were washed off, and inoculated areas were targeted by microbubbles (~0.1-0.5 mm dia.) through a bubble diffuser (1.0 L air/min) for 1, 2, 5, or 10 min. For steel, L. monocytogenes (48 h drying) detachment peaked at 2.95 mean log reduction after 10 min of microbubbles when compared to a no-bubble treatment. After 48 h pathogen drying, cucumbers treated for 10 min showed a 1.78 mean log reduction of L. monocytogenes. For avocados, L. monocytogenes (24 h drying) detachment peaked at 1.65 log reduction after 10 min of microbubbles. Microbubble applications may be an effective, economical, and environmentally friendly way to remove L. monocytogenes, and possibly other bacterial pathogens, from food contact surfaces and the surfaces of whole, intact fresh produce.
Insights
Microbubbles effectively detach Listeria monocytogenes from produce and surfaces. This low-intensity cavitation offers an economical and eco-friendly method for pathogen removal in food safety applications.
Area of Science:
- Food science and technology
- Microbiology
- Environmental engineering
Background:
- Bacterial contamination of fresh produce, like Listeria monocytogenes, poses a significant food safety risk.
- Pathogens can contaminate produce during various stages, including harvesting, packaging, and transport.
- Effective removal of Listeria monocytogenes from food contact surfaces and produce is crucial.
Purpose of the Study:
- To evaluate the efficacy of low-intensity cavitation using microbubbles for detaching Listeria monocytogenes.
- To assess microbubble performance on stainless steel, cucumber, and avocado surfaces.
- To determine optimal microbubble application time for pathogen removal.
Main Methods:
- Inoculation of stainless steel, cucumber, and avocado surfaces with Listeria monocytogenes.
- Application of microbubbles generated by injecting air into water via a bubble diffuser.
- Quantification of pathogen reduction using log reduction values after varying treatment durations.
Main Results:
- Microbubble treatment achieved a peak mean log reduction of 2.95 for Listeria monocytogenes on stainless steel after 10 minutes.
- A mean log reduction of 1.78 for Listeria monocytogenes was observed on cucumbers after 10 minutes of microbubble treatment.
- Avocado surfaces showed a peak mean log reduction of 1.65 for Listeria monocytogenes after 10 minutes of microbubble application.
Conclusions:
- Microbubble cavitation is a promising method for removing Listeria monocytogenes from food contact surfaces.
- This technique demonstrates potential for reducing bacterial pathogens on whole, intact fresh produce.
- Microbubble applications present an effective, economical, and environmentally friendly food safety solution.
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