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Ginsenoside Rb1 from Panax notoginseng Suppressed TNF-α-Induced Matrix Metalloproteinase-9 via the Suppression of

Wen-Tao Sun1, Cindy L H Yang2, Terry C T Or2

  • 1School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao 266071, China.

Molecules (Basel, Switzerland)
|November 26, 2022
PubMed

Insights

Ginsenoside Rb1 from Panax notoginseng inhibits tumor necrosis factor-α-induced matrix metalloproteinase-9 (MMP-9) production. This compound shows potential for treating inflammatory conditions and cancer metastasis.

Area of Science:

  • Pharmacology
  • Biochemistry
  • Traditional Chinese Medicine

Background:

  • Chronic inflammation stimulates matrix metalloproteinases (MMPs), leading to extracellular matrix degradation.
  • Overexpression of MMP-9 (Gelatinase B) is implicated in cardiac remodeling and liver cancer metastasis.
  • Panax notoginseng (Sanqi) is a traditional Chinese herb with known myocardial protective and anti-tumor properties.

Purpose of the Study:

  • To investigate the inhibitory effects of Panax notoginseng extracts on tumor necrosis factor-α (TNF-α)-induced MMP-9 expression.
  • To identify the active compound responsible for MMP-9 inhibition.
  • To explore the therapeutic potential of the identified compound in inflammatory and metastatic conditions.

Main Methods:

  • Bioassay-guided fractionation of Panax notoginseng extracts.
  • Silica gel column chromatography and high-performance liquid chromatography for compound isolation.
  • Identification of the active compound using nuclear magnetic resonance spectroscopy.
  • Assay of MMP-9 production in H9c2 and HepG-2 cells stimulated with TNF-α.

Main Results:

  • Ginsenoside Rb1 was isolated and identified as the active compound.
  • Ginsenoside Rb1 significantly inhibited TNF-α-induced MMP-9 production in both cardiac myoblast H9c2 and liver carcinoma HepG-2 cells.
  • Ginsenoside Rb1 did not affect MMP-2 levels or cell proliferation in either cell line.
  • Inhibitory effects are potentially mediated by modulation of protein kinase and nuclear factor kappa B signaling pathways.

Conclusions:

  • Ginsenoside Rb1 demonstrates potent inhibition of MMP-9 production.
  • This suggests a potential therapeutic role for Ginsenoside Rb1 in managing inflammatory diseases and preventing metastasis of hepatocellular carcinomas.
  • Further research into its signaling pathway modulation could elucidate its mechanisms of action.
Keywords:
Ginsenoside Rb1NF-κBPanax notoginsengdouble-strand RNA-dependent protein kinase (PKR)matrix metalloproteinase 9

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