![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg&w=3840&q=50)
Highly Bright Gold Nanowires Arrays for Sensitive Detection of Urea and Urease
Yan Li1, Aowei Zhao1, Jieqiong Wang1
1College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology of Zhejiang Province, Hangzhou Normal University, Hangzhou 311121, China.
Abstract:
In this work, highly fluorescent gold nanowire arrays (Au NWs) are successfully synthesized by assembling Zn2+ ions and non-emissive oligomeric gold-thiolate clusters using mercaptopropionic acid both as a reducing agent and a growth ligand. The synthesized Au NWs exhibited strong bluish green fluorescence with an absolute quantum yield up to 32% and possessed ultrasensitive pH stimuli-responsive performance in the range of 7.0-7.8. Based on the excellent properties of the as-prepared nanowire arrays, we developed a facile, sensitive, and selective fluorescent method for quantitative detection of urea and urease. The fabricated nanoprobe showed superior biosensing response characteristics with good linearities in the range of 0-100 μM for urea concentration and 0-12 U/L for urease activity. In addition, this fluorescent probe afforded relatively high sensitivity with the detection limit as low as 2.1 μM and 0.13 U/L for urea and urease, respectively. Urea in human urine and urease in human serum were detected with satisfied results, exhibiting a promising potential for biomedical application.
