Highly Sensitive and Selective Detection of Melatonin in Biofluids by Antipyrine Based Fluorophore

A simple fluorescent based organic fluorophore was synthesized and it shows significant fluorescent intensity with melatonin (MLN). Hence, it was applicable to the detection of MLN by colorimetric and fluorimetric techniques at neutral pH. Under optimized experimental condition, the synthesized organic fluorophore detects MLN selectively in the presence of other interfering biomolecules through ICT mechanism. The melatonin sensing mechanism is supported by DFT and ¹H-NMR titration. Based on the findings, this method can be applied to design a simple clinical diagnostic tool for MLN.