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CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction

Baoyi Zhu1, Jun Gao2, Yuying Zhang3

  • 1Department of Urology, The Sixth Affiliated Hospital of Guangzhou Medical University (Qingyuan People's Hospital), B24 Yinquan Road, Qingcheng, Qingyuan, 511500, Guangdong, People's Republic of China.

BMC Urology
|November 26, 2022
PubMed
Abstract

Insights

The circRNA/miRNA/mRNA axis is implicated in the progression of partial bladder outlet obstruction (pBOO). This study identified key regulatory pathways, offering potential new therapeutic strategies for pBOO.

Area of Science:

  • Molecular Biology
  • Genetics
  • Urology

Background:

  • Circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) form regulatory axes crucial in disease pathogenesis.
  • The specific role of the circRNA/miRNA/mRNA axis in partial bladder outlet obstruction (pBOO) remains largely unexplored.
  • Understanding these interactions is vital for elucidating disease mechanisms.

Purpose of the Study:

  • To investigate the complex regulatory mechanisms of the circRNA/miRNA/mRNA axis in the context of pBOO.
  • To identify key molecular players and pathways involved in pBOO pathogenesis.
  • To explore potential therapeutic targets for pBOO.

Main Methods:

  • Establishment of a pBOO rat model and collection of bladder tissues.
  • High-throughput mRNA sequencing to identify differentially expressed mRNAs.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.
  • Competing endogenous RNA (ceRNA) network analysis to predict circRNA/miRNA/mRNA interactions.
  • Quantitative real-time PCR (qRT-PCR) for expression validation.
  • miRanda software for predicting binding sites between RNA molecules.

Main Results:

  • A total of 571 differentially expressed mRNAs were identified in the pBOO group compared to the sham group (286 up-regulated, 285 down-regulated).
  • GO analysis indicated involvement in cellular processes, single-organism processes, and cellular functions.
  • KEGG analysis highlighted enriched pathways including metabolic pathways, cell adhesion molecules (CAMs), and HTLV-I infection.
  • ceRNA network analysis and qRT-PCR validation suggested potential roles for specific circRNA/miRNA/mRNA axes (e.g., chr3:113195876|113197193/rno-miR-30c-1-3p/Gata4).

Conclusions:

  • The circRNA/miRNA/mRNA axis plays a significant role in the progression of pBOO.
  • This research provides novel insights into the pathogenesis of pBOO.
  • The identified regulatory axes may represent promising targets for future pBOO treatment strategies.
Keywords:
Bladder outlet obstructionceRNAcircRNA/miRNA/mRNAmRNA sequencing

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