Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interaction between perfluoro-octanoic sulfonate and common antibiotics induces developmental anomalies and lethality in Xenopus laevis.

Developmental dynamics : an official publication of the American Association of Anatomists·2025
Same author

In preprints: of genitalia and six-legged mice.

Development (Cambridge, England)·2023
Same author

Developmental biology: A 5'Hoxd-Gli3 balance in tetrapod axial polarity.

Current biology : CB·2021
Same author

Limb positioning and initiation: An evolutionary context of pattern and formation.

Developmental dynamics : an official publication of the American Association of Anatomists·2021
Same author

Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression.

Cell reports·2021
Same author

Attenuated Fgf Signaling Underlies the Forelimb Heterochrony in the Emu Dromaius novaehollandiae.

Current biology : CB·2019

Related Experiment Video

Updated: Aug 19, 2025

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

1.1K

Facile methods for reusing laboratory plastic in developmental biology experiments.

Maggie Clancy1, Isabel S Wade1, John J Young1

  • 1Biology Department, Simmons University, Boston, MA, 02115, USA.

Differentiation; Research in Biological Diversity
|November 26, 2022
PubMed
Summary
This summary is machine-generated.

Life science labs can reduce plastic waste by reusing culture dishes. Sterilization with bleach or alcohol effectively cleans dishes without impacting experimental outcomes for Xenopus laevis research.

More Related Videos

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

23.4K
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.7K

Related Experiment Videos

Last Updated: Aug 19, 2025

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

1.1K
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

23.4K
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.7K

Area of Science:

  • Developmental Biology
  • Environmental Science
  • Laboratory Practices

Background:

  • Single-use plastics are a major source of global plastic pollution, particularly in marine environments.
  • Life science laboratories rely on sterile, single-use plastics for experimental reliability, contributing significantly to plastic waste.
  • Developmental biology research generates substantial plastic waste from culture dishes.

Purpose of the Study:

  • To investigate the feasibility of reusing plastic culture dishes in life science laboratories.
  • To propose and test methods for sterilizing and reusing laboratory plastics.
  • To assess the impact of reusing plastic dishes on experimental outcomes and estimate potential waste reduction and cost savings.

Main Methods:

  • Tested the efficacy of bleach and ethyl alcohol for sterilizing used plastic culture dishes.
  • Cultured Xenopus laevis embryos in washed and reused plastic dishes, assessing development and survival rates.
  • Surveyed life science laboratories to estimate waste reduction and economic benefits of plastic reuse.

Main Results:

  • Bleach and ethyl alcohol effectively sterilized used plastic culture dishes.
  • Reusing cleaned plastic dishes did not adversely affect the development or survival of Xenopus laevis embryos.
  • Survey data indicated significant potential for waste reduction and cost savings through plastic reuse.

Conclusions:

  • Reusing plastic culture dishes is a viable strategy to mitigate single-use plastic waste in developmental biology research.
  • Established sterilization methods are effective and do not compromise experimental integrity.
  • Standardizing plastic reuse procedures can lead to economic benefits and promote environmentally conscious laboratory practices.