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Related Experiment Videos

Kininogenase from rat vascular tissue.

H Nolly, A G Scicli, G Scicli

    Advances in Experimental Medicine and Biology
    |January 1, 1986
    PubMed
    Summary
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    A novel kininogenase was isolated from rat vascular tissue, showing characteristics similar to glandular kallikrein. This enzyme releases bradykinin, potentially regulating vascular tone.

    Area of Science:

    • Biochemistry
    • Physiology
    • Pharmacology

    Background:

    • Vascular tissue contains enzymes that may influence vascular tone.
    • Kininogenases are enzymes that release kinins, which are vasoactive peptides.
    • Glandular kallikrein is a known kininogenase with physiological roles.

    Purpose of the Study:

    • To purify and characterize a kininogenase from rat vascular tissue.
    • To compare the properties of the vascular kininogenase with rat urinary kallikrein.
    • To investigate the potential role of vascular kininogenase in regulating vascular tone.

    Main Methods:

    • Partial purification of vascular kininogenase from rat tail arteries and veins.
    • Chromatofocusing, affinity chromatography, and gel filtration for purification.

    Related Experiment Videos

  • Enzyme activity assays, antibody inhibition studies, and gel electrophoresis for characterization.
  • Identification of released kinins using high-performance liquid chromatography.

    • Main Results:

    • A kininogenase was purified from vascular tissue.
    • The vascular kininogenase showed similar properties to glandular kallikrein, including inhibition by specific antibodies and aprotinin.
    • The enzyme released bradykinin, identified by HPLC.
    • Vascular kininogenase and rat urinary kallikrein exhibited similar chromatographic and electrophoretic behavior.

    Conclusions:

    • Rat vascular kininogenase is similar to glandular kallikrein.
    • Locally released kinins by vascular kininogenase may play a role in regulating vascular tone.
    • Further research is warranted to elucidate the precise physiological functions.