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MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling

Xiao-Jing Gu1, Ya-Jun Li2, Fang Wang1

  • 1Department of Gastroenterology, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Prefecture, China.

World Journal of Gastrointestinal Oncology
|November 28, 2022
PubMed
Abstract

Insights

MicroRNA-30e-3p (miR-30e-3p) is downregulated in gastric cancer (GC), suppressing tumor growth and metastasis. The miR-30e-3p/THOC2 axis regulates the PI3K/AKT/mTOR pathway, offering potential new therapies for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer (GC) presents significant global health challenges due to high morbidity and mortality.
  • Understanding the molecular mechanisms driving GC development and metastasis is crucial for therapeutic advancement.
  • MicroRNAs (miRNAs) are increasingly recognized for their roles in regulating cancer progression.

Purpose of the Study:

  • To investigate the expression and functional role of miR-30e-3p in the context of gastric cancer.
  • To identify the molecular targets and pathways regulated by miR-30e-3p in GC.

Main Methods:

  • Quantitative analysis of miR-30e-3p expression in GC tissues and correlation with patient outcomes.
  • Functional assays to assess the impact of miR-30e-3p on GC cell proliferation and metastasis.
  • Bioinformatics prediction and luciferase reporter assays to validate THOC2 as a direct target of miR-30e-3p.
  • Investigation of signaling pathways, including PI3K/AKT/mTOR, affected by the miR-30e-3p/THOC2 interaction.

Main Results:

  • MiR-30e-3p was found to be significantly downregulated in GC, with lower levels correlating with poorer patient prognosis.
  • Overexpression of miR-30e-3p inhibited GC cell growth and metastatic potential.
  • THO complex 2 (THOC2) was confirmed as a direct downstream target of miR-30e-3p.
  • The miR-30e-3p/THOC2 axis was demonstrated to regulate the PI3K/AKT/mTOR signaling pathway in GC cells.

Conclusions:

  • A novel functional axis, miR-30e-3p/THOC2, plays a significant role in the development and progression of gastric cancer.
  • This regulatory axis impacts critical cellular processes via the PI3K/AKT/mTOR pathway.
  • The miR-30e-3p/THOC2 axis represents a promising therapeutic target for developing novel anti-gastric cancer strategies.
Keywords:
Gastric cancerMiR-30e-3pPI3K/AKT/mTOR signalingTHO complex2

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