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Updated: Aug 19, 2025
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Exosomal MicroRNA Profiling
Neda Rahimian1,2, Javid Sadri Nahand3, Michael R Hamblin4
1Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Abstract:
Exosomes are extracellular vesicles, which have the ability to convey various types of cargo between cells. Lately, a great amount of interest has been paid to exosomal microRNAs (miRNAs), since much evidence has suggested that the sorting of miRNAs into exosomes is not an accidental process. It has been shown that exosomal miRNAs (exo-miRNAs) are implicated in a variety of cellular processes including (but not limited to) cell migration, apoptosis, proliferation, and autophagy. Exosomes can play a role in cardiovascular diseases and can be used as diagnostic biomarkers for several diseases, especially cancer. Tremendous advances in technology have led to the development of various platforms for miRNA profiling. Each platform has its own limitations and strengths that need to be understood in order to use them properly. In the current review, we summarize some exo-miRNAs that are relevant to exo-miRNA profiling studies and describe new methods used for the measurement of miRNA profiles in different human bodily fluids.
Insights
Exosomes, tiny vesicles carrying microRNAs (miRNAs), are crucial in cell communication and disease, particularly cancer. This review covers exosomal miRNAs (exo-miRNAs) and profiling methods for bodily fluid analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Exosomes are extracellular vesicles involved in intercellular communication.
- Exosomal microRNAs (exo-miRNAs) play significant roles in cellular processes like migration, apoptosis, proliferation, and autophagy.
- Exo-miRNAs are implicated in diseases such as cardiovascular conditions and cancer, serving as potential diagnostic biomarkers.
Conclusions:
- Exosomal microRNAs (exo-miRNAs) are vital mediators of intercellular signaling with diagnostic potential.
- Accurate and reliable exo-miRNA profiling requires careful selection and understanding of available technological platforms.
- Advancements in profiling technologies facilitate the use of exo-miRNAs as biomarkers in various human bodily fluids.
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