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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Aug 19, 2025

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

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Exosomal MicroRNA Profiling.

Neda Rahimian1,2, Javid Sadri Nahand3, Michael R Hamblin4

  • 1Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2022
PubMed
Summary
This summary is machine-generated.

Exosomes, tiny vesicles carrying microRNAs (miRNAs), are crucial in cell communication and disease, particularly cancer. This review covers exosomal miRNAs (exo-miRNAs) and profiling methods for bodily fluid analysis.

Keywords:
ExosomeIsolationMicroRNA

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Exosomes are extracellular vesicles involved in intercellular communication.
  • Exosomal microRNAs (exo-miRNAs) play significant roles in cellular processes like migration, apoptosis, proliferation, and autophagy.
  • Exo-miRNAs are implicated in diseases such as cardiovascular conditions and cancer, serving as potential diagnostic biomarkers.

Conclusions:

  • Exosomal microRNAs (exo-miRNAs) are vital mediators of intercellular signaling with diagnostic potential.
  • Accurate and reliable exo-miRNA profiling requires careful selection and understanding of available technological platforms.
  • Advancements in profiling technologies facilitate the use of exo-miRNAs as biomarkers in various human bodily fluids.