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Related Experiment Video

Updated: Aug 19, 2025

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

MicroRNA Profiling Using a PCR-Based Method.

Giuliana A de Ferronato¹, Marcela B Cerezetti¹, Alessandra Bridi¹

¹Department of Veterinary Medicine, College of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)

|November 28, 2022

Summary

This summary is machine-generated.

This study details a primer-based quantitative reverse transcription PCR (RT-qPCR) protocol for analyzing microRNA (miRNA) expression. This method offers a sensitive, specific, and cost-effective approach for miRNA profiling and potential therapeutic applications.

Keywords:

Gene expression MicroRNAs Molecular biology Post-transcriptional modulation RT-qPCR protocol

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Last Updated: Aug 19, 2025

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

MicroRNAs (miRNAs) are small non-coding RNA molecules regulating gene expression post-transcriptionally.
miRNA profiling aids in understanding biological pathways, molecular diagnostics, and therapeutic strategies.
Established methods for miRNA analysis include RT-qPCR, microarrays, and RNA sequencing.

Purpose of the Study:

To provide a detailed, step-by-step protocol for miRNA analysis using primer-based RT-qPCR.
To highlight RT-qPCR as a sensitive, specific, and cost-effective alternative for miRNA expression studies.

Main Methods:

Detailed description of a primer-based RT-qPCR protocol for miRNA quantification.
Emphasis on the practical implementation of the RT-qPCR technique for miRNA analysis.

Main Results:

The protocol enables precise measurement of miRNA expression levels.
Demonstrates the feasibility and advantages of using RT-qPCR for miRNA profiling.

Conclusions:

Primer-based RT-qPCR is a valuable and accessible method for miRNA expression analysis.
This protocol facilitates research in miRNA-related biological processes and diagnostics.