Sorting and Screening of Quaternary Ammonium Lipoids for Membrane-Binding Assays Based on Electrochemiluminescent...

Being synthetic supplements to natural lipids, lipoids now play an increasingly significant role in nanopore sequencing, olfactory sensing, and nanoimpact electrochemistry. Yet, systematic comparisons to sort and screen qualified lipoids are lacking for specific scenario applications. Here, taking the merits of electrochemiluminescence (ECL) in probing biointerfacial events, a new metric was proposed for the evaluation of substrate candidacy in the pool of hyamine bromides (ABs), that are used to cohere with electron-rich porphyrins for deep eutectics-like ECL matrices. Using a state-of-the-art framework emitter, the cocrystalline nanosheet of C₇₀ and zinc meso-tetraphenylporphine (ZnTPP) via simple liquid-liquid interfacial deposition, 6 out of 20 ABs were inspected and identified as not only amenable filmogens but excitonic sensitizers in key terms of ECL strength as well as voltammetric characteristics. Among them, the methyltrioctyl (MTOAB) headgroup stood out; while the ECL activity at ZnTPP-C₇₀@MTOAB was proven to be dictated by ionophoresis across multilamellar lipoidal layers. Thus, target-induced membrane deformation would let coreactant scavengers in to quench ECL, which enabled assays on two less visited bioprocesses regarding (1) the lipid solubility of ipratropium bromide, an aerosol medication for rhinitis treatment; and (2) the resorption of selenosugar as the central metabolite of Se-proteins on kidney glomerular basement barrier. Both resulted in nice membrane-binding measurements with comparable dissociation constants to reported microfluidic ELISA methods. By and large, though still being rudimentary, such parametrization of ECL-able biofilm would set up a basic ECL toolbox for archiving and resourcing multilipoidal even lipid-lipoid combos to handle the realistic (sub)cytomembrane processes in the future.