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Updated: Aug 19, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
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Large-scale expanded sample imaging with tiling lattice lightsheet microscopy.

Chieh-Han Lu1, Cheng-Yu Huang2, Xuejiao Tian3

  • 1Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan; Institute and Undergraduate Program of Electro-Optical Engineering, National Taiwan Normal University, Taipei 116, Taiwan.

The International Journal of Biochemistry & Cell Biology
|November 28, 2022
PubMed
Summary
This summary is machine-generated.

Expansion microscopy (ExM) enables super-resolution imaging. Tiling lattice lightsheet microscopy (tLLSM) enhances deep 3D imaging for ExM applications, improving visualization of biological nanostructures.

Keywords:
3D imagingExpansion microscopyLightsheet microscopySuper-resolution imagingThick tissue imagingTiling lattice lightsheet

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Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • Observing biological nanostructures is crucial for understanding cellular functions.
  • Expansion microscopy (ExM) allows super-resolution visualization of biological samples using conventional light microscopes.
  • Physical expansion in ExM necessitates advanced deep and precise 3D imaging techniques.

Purpose of the Study:

  • To explain the principles of tiling lattice lightsheet microscopy (tLLSM).
  • To discuss the applications of tLLSM in conjunction with ExM.
  • To highlight tLLSM's capability for high-precision 3D imaging of expanded biological samples.

Main Methods:

  • Utilizes lattice lightsheet microscopy (LLSM) for planar illumination confined within high numerical aperture objectives.
  • Incorporates optical tiling to expand the field of view (FoV) without mechanical sample or objective movement.
  • Combines LLSM with optical tiling to create tLLSM for enhanced 3D imaging.

Main Results:

  • LLSM provides deep and precise 3D imaging capabilities suitable for ExM.
  • Optical tiling within LLSM effectively increases the field of view for comprehensive imaging.
  • tLLSM integrates these features for advanced visualization of expanded biological samples.

Conclusions:

  • tLLSM is a powerful technique for high-precision 3D imaging of samples prepared with ExM.
  • This method addresses the increased imaging demands posed by physically expanded biological specimens.
  • tLLSM facilitates a deeper understanding of biological nanostructures and their functions.