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Differential mRNA Expressions in HCMV infected HUVECs
Chang Ning Lyu1, Ji Chen Li2, Qi An1
1School of Clinical Medicine, Weifang Medical University, Weifang 261053, Shandong, China.
Biomedical and Environmental Sciences : BES
|November 28, 2022
Summary
This study identified gene expression changes in human cytomegalovirus (HCMV)-infected endothelial cells, revealing key pathways involved in infection and potential atherosclerosis mechanisms.
Area of Science:
- Molecular Biology
- Virology
- Cardiovascular Research
Background:
- Human cytomegalovirus (HCMV) infection is linked to various diseases, including atherosclerosis.
- Endothelial cells play a critical role in vascular health and are a target for HCMV.
Purpose of the Study:
- To identify gene expression profiles of HCMV-infected human umbilical vein endothelial cells (HUVECs).
- To elucidate the molecular mechanisms underlying HCMV-induced pathogenesis of atherosclerosis.
Main Methods:
- Microarray technology was employed to analyze gene expression differences in HCMV-infected HUVECs.
- Quantitative real-time PCR (qPCR) was used to validate transcriptome findings.
- Bioinformatic analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, were performed.
Main Results:
- A total of 2,583 differentially expressed genes were identified (2,176 up-regulated, 407 down-regulated).
- Enrichment analyses highlighted involvement in protein kinase activity, inflammatory response, ubiquitination, protein phosphorylation, cell metabolism, and exosomes.
- Protein-protein interaction (PPI) analysis identified 12 key genes, validated by qPCR, showing consistency with microarray data.
Conclusions:
- HCMV infection significantly alters gene expression in endothelial cells, impacting pathways crucial for vascular function.
- The identified genes and pathways provide insights into the molecular mechanisms of HCMV infection and its contribution to atherosclerosis development.
