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Related Experiment Video

Updated: Aug 19, 2025

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Mesenchymal Stem Cell Identification After Delayed Cord Clamping.

Emily R Smith1,2, William M Curtin3,4, Kevin P Yeagle5

  • 1Division of Maternal-Fetal Medicine, Department of Obstetrics, Penn State College of Medicine, Penn State Health, Milton S. Hershey Medical Center, Hershey, PA, USA.

Reproductive Sciences (Thousand Oaks, Calif.)
|November 28, 2022
PubMed
Summary
This summary is machine-generated.

Delayed cord clamping allows for the identification and quantification of mesenchymal stem cells (MSCs) in umbilical cord blood (UCB) from both preterm and term infants. This study found no significant difference in MSC numbers between the two groups, suggesting feasibility for therapeutic applications.

Keywords:
Delayed cord clampingFlow cytometryMesenchymal stem cellsUmbilical cord blood

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Area of Science:

  • Stem Cell Biology
  • Neonatal Medicine
  • Hematology

Background:

  • Mesenchymal stem cells (MSCs) possess immunomodulatory and regenerative properties.
  • Umbilical cord blood (UCB) is a rich source of MSCs.
  • Delayed cord clamping (DCC) is increasingly practiced in preterm and term births.

Purpose of the Study:

  • To assess the feasibility of identifying and quantifying MSCs in UCB collected after DCC in preterm and term neonates.
  • To compare the yield of MSCs between preterm and term UCB samples obtained via DCC.

Main Methods:

  • UCB samples (3 mL) were collected from preterm and term infants following DCC.
  • MSCs were isolated using density gradient centrifugation and magnetic bead selection (CD34+ depletion).
  • Flow cytometry was employed to identify and quantify MSCs using specific cell surface markers (CD29, CD44, CD73, CD105) and exclude hematopoietic cells (CD45).

Main Results:

  • MSCs were successfully identified and quantified in 10 out of 12 UCB samples (83%).
  • The median percentage of MSCs in viable UCB mononuclear cells was 0.0174%, with no significant difference between term (0.148%) and preterm (0.116%) samples (p=0.17).
  • No significant difference was observed in the median absolute number of MSCs between term (3384) and preterm (36) samples (p=0.12).

Conclusions:

  • Delayed cord clamping provides a feasible method for obtaining UCB suitable for MSC identification and quantification.
  • The number of MSCs in UCB does not significantly differ between preterm and term infants following DCC.
  • These findings support the potential of UCB collected after DCC as a source for MSC-based therapies.