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Aspergillus flavus genetic structure at a turkey farm
Ghaya Cherif1,2, Ines Hadrich3, Myriam Harrabi2,4
1Laboratory of Parasitology and Mycology UR17SP 03, La Rabta Hospital, Tunis, Tunisia.
Environmental and avian Aspergillus flavus (A. flavus) populations show distinct genetic structures. This suggests genome-based adaptation in A. flavus isolates causing avian aspergillosis in poultry.
Area of Science:
- Mycology
- Veterinary Pathology
- Population Genetics
Background:
- Aspergillus flavus is a common fungus found in the environment.
- It is also a significant cause of life-threatening infections in birds.
Purpose of the Study:
- To investigate the genetic diversity and population structure of A. flavus.
- Samples were collected from turkey lungs and the farm environment.
Main Methods:
- Isolates were identified using morphology and ITS sequencing.
- Multilocus microsatellite genotyping was performed with six markers.
- Population genetic indices were calculated using FSTAT and STRUCTURE.
Main Results:
- Environmental A. flavus isolates (n=63) had 36 genotypes (diversity=0.57).
- Avian A. flavus isolates (n=19) had 17 genotypes (diversity=0.89).
- Environmental and avian populations showed distinct genetic structures, with evidence of gene flow.
Conclusions:
- Environmental and avian A. flavus populations exhibit different genetic structures.
- This indicates adaptation at the genomic level for isolates causing avian aspergillosis.
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