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Real-Time Objective Evaluation of the Ischemic Stroke through pH-Responsive Fluorescence Imaging
Shasha Yao1,2, Chengbin He3, Pengcheng Yuan1,2
1Key Laboratory of Smart Biomaterials of Zhejiang Province, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China.
Advanced Healthcare Materials
|November 29, 2022
Summary
This study introduces a new fluorescent probe for real-time assessment of ischemic stroke (IS) severity. The probe visualizes the acidic microenvironment, offering a novel method for objective evaluation and insight into IS progression.
Area of Science:
- Biomedical Imaging
- Neuroscience
- Materials Science
Background:
- Accurate ischemic stroke (IS) assessment is crucial for timely and effective treatment.
- Current diagnostic methods like CT and MRI have limitations in real-time pathological and microenvironmental evaluation.
- Near-infrared fluorescence imaging offers high sensitivity, resolution, and real-time capabilities for IS imaging.
Purpose of the Study:
- To develop a pH-responsive fluorescent probe for in vivo visualization of IS severity.
- To establish a correlation between fluorescence signals and neurological deficit scores in IS.
- To provide a novel visualized method for objective IS evaluation and understand the role of acidic microenvironments in IS progression.
Main Methods:
- Preparation of a pH-responsive fluorescent liposomal probe (BOD@Lip).
- In vivo fluorescence imaging to visualize the acidic microenvironment in IS.
- Correlation analysis between fluorescence signals and established neurological deficit scores.
Main Results:
- The BOD@Lip probe successfully visualized the degree of IS in real-time.
- A significant correlation was established between fluorescence signals from the probe and neurological deficit scores.
- The study demonstrated the utility of the probe in assessing the acidic microenvironment associated with IS.
Conclusions:
- A novel pH-responsive fluorescent probe (BOD@Lip) enables objective, real-time assessment of ischemic stroke severity.
- This imaging approach provides new insights into the relationship between acidic microenvironments and IS progression.
- The findings offer a promising visualized route for evaluating IS and guiding clinical treatment.
