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Updated: Aug 19, 2025
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
miR-1322 protects against the myocardial ischemia via LRP8/PI3K/AKT pathway
1Hebei Renbo Technology Co. LTD, China; Beijing Xinshengyuan Biomedical Technology Co., LTD, China.
Introduction:
Myocardial infarction is a fatal disease that causes millions of deaths worldwide every year. The damage and recovery of cardiomyocytes are closely related to changes in gene expression. miRNA may be a new therapeutic target of myocardial ischemia-reperfusion.
Methods:
The differential expression genes were analyzed based on GSE83500, GSE60993 and GSE154733. miRNA expression profile data and clinical data were downloaded from GSE76591. Bioinformatics analysis including limma package, cluster analysis, WGCNA analysis were performed. H9c2 cell hypoxia model and mouse myocardial ischemia model were established. Q-PCR, Western blot and luciferase assay were carried out.
Results:
miR-1322 was identified as a significantly differentially expressed miRNA in myocardial ischemi. Yin Yang 1(YY1) was significantly highly expressed in cells with hypoxia treatment (P < 0.05), and myocardial ischemia mice (P < 0.01), which was identified as the transcription factor of miR-1322. The protein expression of LRP8 was lower in cells with hypoxia treatment and myocardial ischemia mice (P < 0.05) and LRP8 was the target gene of miR-1322. The overexpression of LRP8 could significantly increase the expression of p-PI3K, p-AKT, and P70 S6K (P < 0.05). LRP8 regulated PI3K/AKT/P70 S6K signaling pathway, eventually resulting in cell apoptosis.
Conclusion:
Our results suggested that miR-1322 can protect against the myocardial ischemia via LRP8/PI3K/AKT pathway.
Insights
MicroRNA-1322 (miR-1322) protects against myocardial ischemia by regulating the LRP8/PI3K/AKT pathway. This finding offers a potential therapeutic target for heart attack recovery and treatment.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Gene Regulation
Background:
- Myocardial infarction is a leading cause of global mortality.
- Cardiomyocyte damage and recovery are linked to gene expression changes.
- MicroRNAs (miRNAs) show potential as therapeutic targets for myocardial ischemia-reperfusion injury.
Purpose of the Study:
- To investigate the role of microRNAs in myocardial ischemia-reperfusion.
- To identify specific miRNAs and their regulatory pathways involved in heart attack.
- To explore potential therapeutic strategies targeting miRNA in cardiovascular disease.
Main Methods:
- Bioinformatic analysis of gene expression datasets (GSE83500, GSE60993, GSE154733, GSE76591).
- Establishment of H9c2 cell hypoxia and mouse myocardial ischemia models.
- Validation using quantitative real-time PCR (Q-PCR), Western blot, and luciferase assays.
Main Results:
- miR-1322 was identified as a significantly differentially expressed miRNA in myocardial ischemia.
- Yin Yang 1 (YY1) was identified as the transcription factor for miR-1322.
- LRP8 was confirmed as a target gene of miR-1322, and its overexpression activated the PI3K/AKT/P70S6K pathway, influencing cell apoptosis.
Conclusions:
- miR-1322 plays a protective role in myocardial ischemia.
- The protective effect is mediated through the LRP8/PI3K/AKT signaling pathway.
- miR-1322 represents a promising therapeutic target for myocardial ischemia-reperfusion injury.
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