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Updated: Aug 19, 2025
Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Published on: November 28, 2019
Cellulose-based fluorescent materials fabricated in CO2 switchable solvent for freshness monitoring
1School of Food Science and Pharmaceutics, Zhejiang Ocean University, No. 1 Haida South Road, Lincheng Changzhi Island, Zhoushan, Zhejiang 316000, China; Key Laboratory of Bio-based Polymeric Materials Technology and Application of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, 1219 Zhongguan West Road, Zhenhai, Ningbo, Zhejiang 315201, China.
Abstract:
Herein, a novel cellulose-based fluorescent material with amine-responsive property was developed in a CO2 switchable solvent for the contactless, real-time, and visual monitoring of the freshness of shrimp, crab, and fish. A nucleophilic cellulose carbonate intermediate is in-situ formed upon dissolution of cellulose in the CO2 switchable solvent, which is adopted for the highly effective grafting of poly(l-lactic acid) and amine-responsive fluorescent reagent 7-amino-4-(trifluoromethyl) coumarin onto the cellulose backbone via ring opening polymerization and nucleophilic substitution reaction, respectively. The product has good processibility, and sensitive and reversible fluorescent responsive towards ammonia. The fluorescent intensity of the material decreased significantly and its fluorescent color changed from blue to cyan, when the aquatic products got spoiled. This study indicates that the CO2 switchable solvent has great potential for the modification of cellulose with various functionality by taking advantage of the highly active nucleophilic cellulose carbonate intermediate.
