Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infertility in Males01:23

Infertility in Males

315
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
315
Spermatogenesis01:41

Spermatogenesis

102.9K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Normal spermatogenesis in older men is associated with compensatory transcriptome changes.

GeroScience·2026
Same author

Pervasive relaxed selection on spermatogenesis genes coincident with the evolution of polygyny in gorillas.

eLife·2026
Same author

Translation and cultural adaptation of the GENDER-Q with pilot implementation among patients undergoing masculinizing chest surgery.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

Clinical decision support in hematological malignancies using a case-grounded AI agent.

Nature medicine·2026
Same author

Can Hormonal Therapy Improve the Outcomes of mTESE in Patients With Non-Obstructive Azoospermia?

Andrology·2026
Same author

Assessing a Model for a Complex Systemic Disorder-The Value of Male Mice With a Supernumerary X Chromosome for Klinefelter Syndrome Research.

Andrology·2026

Related Experiment Video

Updated: Aug 19, 2025

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.1K

Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns.

Lara M Siebert-Kuss1, Henrike Krenz2, Tobias Tekath2

  • 1Centre of Reproductive Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of Münster, Münster, Germany.

Life Science Alliance
|November 29, 2022
PubMed
Summary

This study maps gene expression during sperm development, revealing thousands of differences between cell types. These findings identify new markers and potential causes for male infertility.

More Related Videos

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

2.2K

Related Experiment Videos

Last Updated: Aug 19, 2025

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.1K
Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
08:21

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

5.9K
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

2.2K

Area of Science:

  • Reproductive Biology
  • Genetics
  • Molecular Biology

Background:

  • Spermatogenesis, the process of sperm development, is crucial for male fertility.
  • Gene expression dysregulation is implicated in male infertility.
  • Detailed understanding of gene expression dynamics during spermatogenesis remains incomplete.

Purpose of the Study:

  • To comprehensively profile gene expression during spermatogenesis.
  • To identify novel germ cell-specific markers.
  • To uncover genes with misregulated expression contributing to male infertility.

Main Methods:

  • Systematic comparison of testicular transcriptomes.
  • Analysis of germ cell-specific whole transcriptome profiles.
  • Identification of differentially expressed genes across successive germ cell differentiation stages.

Main Results:

  • Thousands of differentially expressed genes identified between successive germ cell types.
  • Discovery of novel germ cell-specific markers (e.g., TSPY4, LUZP4, HMGB4).
  • Identification of putatively misregulated genes in male infertility (e.g., RWDD2A, CCDC183).
  • Detection of thousands of genes with germ cell-specific isoforms.

Conclusions:

  • The generated dataset and analytical approach provide valuable insights into male infertility.
  • This work aids in elucidating the genetic and transcriptional underpinnings of male infertility.
  • Identified markers and genes can facilitate future diagnostic and therapeutic strategies.