Related Experiment Video
Updated: Aug 19, 2025

Ubiquitin Chain Analysis by Parallel Reaction Monitoring
Published on: June 17, 2020
Chemical Synthesis of Non-hydrolyzable Ubiquitin(-Like) Hybrid Chains
David A Pérez Berrocal1, Gerbrand J van der Heden van Noort1, Monique P C Mulder2
1Oncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands.
Abstract:
Hybrid chains are a combination of ubiquitin (Ub) and Ub-like (UbL) proteins, expanding on the finely tuned Ub code. To decipher this intricate code, understanding of its assembly, architecture, as well as specific interactors of these Ub/UbL hybrid chains are important, warranting the development of suitable reagents. Here, we describe the chemical methodology to access linkage specific non-hydrolyzable Ub-NEDD8-based chains endowed with an affinity handle in all possible combinations of K48 hybrid chain dimers between Ub and NEDD8.
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