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Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

This protocol describes the culturing, harvesting, and concentration of secreted proteins of Mycobacterium tuberculosis. Culture filtrate proteins (CFPs) are quantified, separated into 20 fractions by isoelectric focusing, and further resolved into 30 fractions using a preparative SDS-PAGE and an electroelution system.
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Institutional Investors01:25

Institutional Investors

Institutional investors play a significant role in financial markets due to the large volume of assets they manage. These include pension funds, mutual funds, insurance companies, and hedge funds. Behavioral finance, which studies how psychological factors influence financial decisions, offers unique insights into their behavior and decision-making processes.While institutional investors are generally perceived as rational and well-informed, they are not immune to behavioral biases. Herding...
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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence10:06

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine...
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An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei09:02

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of...
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The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis23:06

The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis

The microscopic-observation drug-susceptibility (MODS) assay is a low-cost, low-tech tool for high-performance detection of tuberculosis (TB) and multidrug-resistant tuberculosis (MDRTB). This video describes the MODS liquid media culture...
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Institutional Markets01:29

Institutional Markets

Organizations in healthcare, education, and government sectors typically make large-scale purchases and have specific buying needs driven by various regulations, standards, and long-term objectives. Understanding the characteristics and behaviors of institutional buyers is crucial for marketers aiming to engage with this segment effectively.
Characteristics of Institutional Markets
Institutional markets are characterized by their size and purchasing power. Institutions often procure goods and...
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