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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Related Experiment Video

Updated: Aug 19, 2025

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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Improved Sendai viral system for reprogramming to naive pluripotency.

Akira Kunitomi1,2, Ryoko Hirohata1,3, Vanessa Arreola2

  • 1Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan.

Cell Reports Methods
|November 30, 2022
PubMed
Summary

A modified Sendai virus (SeV) vector system generates transgene-free naive human induced pluripotent stem cells (iPSCs) from various somatic cells. These iPSCs exhibit enhanced differentiation potential for regenerative medicine and developmental research.

Keywords:
LMYCSendai virus vectorextra-embryonic trophectodermfeeder-free culturehsa-microRNA-367induced pluripotent stem cellsnaive pluripotencyreprogrammingresidual transgenestemperature sensitivity

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Area of Science:

  • Stem Cell Biology
  • Molecular Virology
  • Regenerative Medicine

Background:

  • Naive human induced pluripotent stem cells (iPSCs) are typically generated using Sendai virus (SeV) vectors, but this method is limited to fibroblasts and requires feeder cells.
  • Conventional SeV vectors are persistent, potentially hindering iPSC differentiation capabilities.

Purpose of the Study:

  • To develop a modified SeV vector system for generating transgene-free naive human iPSCs with improved differentiation potential.
  • To enable iPSC generation from diverse somatic cell types in a feeder-free environment.

Main Methods:

  • Utilized a modified Sendai virus (SeV) vector for reprogramming somatic cells.
  • Established feeder-free culture conditions for iPSC generation.
  • Assessed the differentiation potential of generated iPSCs into trilineage and extra-embryonic trophectoderm.

Main Results:

  • Successfully generated transgene-free naive human iPSCs from fibroblasts and other somatic cell types.
  • The modified SeV vectors were rapidly cleared, allowing for feeder-free iPSC generation.
  • iPSCs derived from the modified method demonstrated superior differentiation capacity compared to conventional methods.

Conclusions:

  • The modified SeV vector system offers a more versatile and efficient method for generating naive human iPSCs.
  • This advancement holds significant promise for applications in early human development research and regenerative medicine.