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Related Experiment Videos

Structure of the lambda att sites generated by int-dependent deletions.

R H Hoess, A Landy

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1978
    PubMed
    Summary

    Bacteriophage lambda integrates into E. coli via site-specific recombination. Altered att sites reveal that only the common core region and phage arms determine recombination specificity.

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    Coming or going it's another pretty picture for the lambda-Int family album.

    Proceedings of the National Academy of Sciences of the United States of America·1999

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Bacteriophage lambda integrates into the Escherichia coli chromosome using site-specific recombination.
    • This process involves specific att sites on both the phage and bacterial chromosomes.

    Purpose of the Study:

    • To determine the nucleotide sequence of altered lambda att sites after site-specific recombination.
    • To re-evaluate the genetic properties and structural elements governing this recombination process.

    Main Methods:

    • Determined the nucleotide sequence of four altered lambda att sites.
    • Analyzed int-dependent deletions within the phage att site.
    • Compared new DNA sequences with homologous regions in phage and bacterial att sites.

    Main Results:

    • Identified alterations in the 15-base-pair common core region of the att sites.
    • Discovered that newly introduced DNA sequences (delta and delta') lack homology with existing att site arms (P, P', B, B').
    • Found that altered att sites retain genetic properties for site-specific recombination.

    Conclusions:

    • The common core region (attP core) and phage arms (P and P') are the sole specificity determinants in int-dependent site-specific recombination.
    • A reinterpretation of att site genetic properties is necessary based on these findings.

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