Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From IMU Streams to Real-Time Decisions: Past-Only Next-Window Badminton Action Prediction.

Sensors (Basel, Switzerland)·2026

Same author

An endoplasmic reticulum-directed ROS burst strategy powered by H<sub>2</sub>O<sub>2</sub> abundance for chemodynamic/immuno tumor therapy.

Chemical communications (Cambridge, England)·2026

Same author

Informed Consent Disclosures and Minimum Requirements in AI Clinical Trials: Cross-Sectional Analysis.

Journal of medical Internet research·2026

Same author

Revascularization of Transcortical Vessels Improves Tendon-to-Bone Healing for Rotator Cuff Repair.

The American journal of sports medicine·2026

Same author

Controlled sweat generation via ultrasound stimulation integrated in a wearable device.

Nature communications·2026

Same author

Portable Electrochemiluminescence Microarray Sensor for Machine Learning-Assisted Quantitative Analysis of Zearalenone.

Analytical chemistry·2026

Same journal

Double-Strand Gated Biosensor for Ultrasensitive T4 PNK Detection via λ-Exonuclease-Driven Background Suppression and Dimer G-Triplex Signal Amplification.

ACS sensors·2026

Same journal

Junction-Amplified Porous SnO<sub>2</sub>-Co<sub>3</sub>O<sub>4</sub> Nanospheres for ppb-Level Low-Temperature Acetone Detection and Wearable-Integrated Breath Monitoring.

ACS sensors·2026

Same journal

A Wearable Earplug-Shaped Piezoresistive Sensor Based on Ear Canal Deformation for the Screening of Temporomandibular Joint Motion Abnormalities.

ACS sensors·2026

Same journal

A Triple-Signal Output Lateral Flow Platform Leveraging CRISPR/Cas12a and Nanozyme Cascades for Ultra-Sensitive Aflatoxin B1 Detection.

ACS sensors·2026

Same journal

Dual-Active-Site Synergy in Metal-Organic Framework-Derived Er:CeO<sub>2</sub>/ZnO Nanofibers Enabling Humidity-Independent Triethylamine Detection at Room Temperature.

ACS sensors·2026

Same journal

A Point-of-Care System for the Quantification of Small-Molecule Drugs in Blood.

ACS sensors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 19, 2025

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

Programmable Microparticle Array for In Situ Modification and Multiple miRNA Detection.

Qinglin Zhu¹, Tingxiu Yan¹, Yuemeng Yang¹

¹Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing100083, People's Republic of China.

|November 30, 2022

Summary

This summary is machine-generated.

This study introduces a programmable microparticle-array acoustic microchip for simultaneous detection of multiple microRNAs (miRNAs) for early cancer diagnosis. The device offers precise detection of pancreatic cancer biomarkers, improving diagnostic accuracy.

Keywords:

biosensing cancer diagnoses in situ analysis microparticle array multiple biomarkers simultaneous detection

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Aug 19, 2025

Performing Custom MicroRNA Microarray Experiments

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Area of Science:

Biomedical Engineering
Molecular Diagnostics
Microfluidics

Background:

Simultaneous detection of multiple microRNAs (miRNAs) is crucial for accurate early cancer diagnosis, reducing misdiagnosis rates.
Current diagnostic methods may lack the sensitivity and specificity required for early-stage cancer detection.
Developing advanced microfluidic platforms is essential for multiplexed biomarker analysis.

Discussion:

A novel programmable microparticle-array-based acoustic microchip was developed for in situ simultaneous detection of multiple miRNAs.
The microchip utilizes piezoelectric transducers to arrange microparticle arrays for enhanced molecular contact and multipoint data correction, reducing false positives.
This platform enables precise fluorescence sensing of multiple target miRNAs, as demonstrated by the detection of pancreatic cancer biomarkers (miRNA-21, miRNA-210, miRNA-155).

Key Insights:

The developed acoustic microchip achieved detection limits of 139.1 pM for miRNA-21, 179.9 pM for miRNA-210, and 111.4 pM for miRNA-155.
The device's programmability via frequency and voltage adjustment allows for flexible biomarker enrichment and detection.
Ultrasound force release enables easy collection of target biomarkers for further analysis.

Outlook:

This technology holds significant potential for improving the accuracy and efficiency of cancer clinical diagnosis through multiplexed miRNA detection.
Further research can explore the application of this platform for other diseases and a broader range of biomarkers.
Optimization of the microchip design and detection parameters could lead to even lower detection limits and enhanced clinical utility.