Enhanced fermentative hydrogen production from potato waste by enzymatic pretreatment

Biological pretreatment and enzymatic hydrolysis have a potential role in the economic production of sugars and fuels from starch biomass. In this study, the Inoculum/Substrate (I/S) ratio effect and enzymatic pretreatments of potato peels for biohydrogen production in batch reactors were investigated. Two enzymes, α-Amylase and Cellulase, were tested separately and coexistent. Results showed that enzymatic hydrolysis using α-Amylase in mesophilic conditions enhanced carbohydrate concentration from 24.10 g/L to 53.47 g/L, whereas, the use of Cellulase and equi-volumetric mixture of both tested enzymes resulted in 47.16 and 48.16 g/L, respectively. The maximum biohydrogen cumulative production of 263 mL (equivalent to 430.37 mL H₂/gVS_added) was obtained using the optimum I/S ratio of 1/6 gVS/gVS at pH 5.5 and incubation temperature of 55°C after 20 days of dark fermentation of potato waste without enzymatic treatment. Under the same operating conditions of the I/S ratio, pH, temperature and the best enzymatic treatment (3 h of substrate enzymatic hydrolysis by α-Amylase), the maximum yield of biohydrogen was 1088 mL (1780.39 mL H₂/gVS_added). The enzymatic hydrolysis method adopted in this study can make overall biohydrogen production an effective process. The modified Gompertz model was found to be an adequate fit for biohydrogen production.