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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Aug 19, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatially resolved proteomics via tissue expansion.

Lu Li^1,2,3,4,5, Cuiji Sun^1,2,4, Yaoting Sun^1,2,3,4

¹Research Center for Industries of the Future and School of Life Sciences, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, 310030, China.

Nature Communications

|November 30, 2022

Summary

This summary is machine-generated.

Expansion Proteomics (ProteomEx) offers a new method for mapping tissue proteome heterogeneity. This technique allows for detailed spatial proteomic analysis of mammalian tissues without specialized equipment.

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Related Experiment Videos

Last Updated: Aug 19, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Area of Science:

Biochemistry
Proteomics
Molecular Biology

Background:

Spatially resolved proteomics is crucial for understanding tissue heterogeneity.
Current methods face challenges in tissue microsampling and mass spectrometry analysis of small volumes.

Purpose of the Study:

To introduce Expansion Proteomics (ProteomEx), a novel method for spatially resolved proteomics.
To enable quantitative profiling of proteome spatial variability in mammalian tissues.

Main Methods:

Combines tissue expansion with mass spectrometry-based proteomics.
Utilizes manual microsampling without specialized equipment.
Achieves ~160 µm lateral resolution, analyzing volumes as small as 0.61 nL.

Main Results:

Demonstrated utility of ProteomEx for large-scale proteomics profiling in brain, liver, and breast cancer tissues.
Successfully applied ProteomEx to identify proteins associated with Alzheimer's disease in mouse brain subregions.

Conclusions:

ProteomEx provides a streamlined and accessible approach for spatially resolved proteomics.
This method facilitates the study of proteome heterogeneity and disease-associated proteins in complex biological samples.