Mitigative effects of dehydrodiisoeugenol on enteritis and co-occurring dysmotility in murine model

The actions and mechanisms of dehydrodiisoeugenol (DEH) on releasing clinical symptoms such as diarrhea caused by inflammatory bowel diseases or colorectal cancer is still unclear. The main purpose is to reveal the mechanism and describe the impacts of DEH on enteritis and accompanying intestinal dysmotility in murine model. The animal model of diarrhea was established through being given acetic acid by intracolonic instillation and restraint stress and the weight of the diarrhea mouse, diarrhea index (the product of stool rate and stool grade) evaluation and then, myeloperoxidase (MPO) activity were determined after administrated with DEH. Meanwhile, the expression of myosin light chain kinase (MLCK) was research by WB method. Moreover, the isolated jejunal segment (IJS) of rats was separated from the intact jejunum and the contractility was measured through BL-420F physiological recording system. DEH could significantly inhibit the intestinal transit in normal mice or diarrhea-predominated mice and reduce the diarrhea index and the level of MPO in mice. DEH concentration-dependently inhibited motility of IJS in different states. DEH significantly markedly ameliorated the histopathology condition and reduce the MLCK expression in acetic acid induced diarrhea mice. DEH simultaneously improved enteritis and co-occurring dysmotility in diarrhea mice characterized by reducing the contractility and MLCK contents in acetic acid induced diarrhea mice.