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Biohydrogen production potential with sulfate and nitrate removal by heat-pretreated enriched sulfate-reducing
1School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar, 382030, India.
Abstract:
In this study, heat-pretreated sulfate-reducing bacteria (SRBs) were evaluated for simultaneous sulfate and nitrate removal in a bioelectrochemical system (BES). The effect of the applied potential of 20 mV to SRBs was evaluated at a sulfate concentration of 3 g/L and/or nitrate concentration of 0.5 g/L supplemented before heat pretreatment for sulfate and nitrate removal. The highest H2 production of 2.24 ± 0.04 mM/L in heat-pretreated culture was observed in the presence of sulfate at an applied potential of 20 mV (BHE-S). Simultaneous reduction of sulfate and nitrate was significant in BESs supplemented with either sulfate or nitrate during heat-shock pretreatment of the culture. The highest SO42- removal of 88.91 ± 0.8% was found in culture heat pretreated with NO3- and applied with 20 mV potential (BHE-N). The kinetics of heat-pretreated culture showed higher R2 and ultimate potential for H2 on the continuous application of 20 mV potential.
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