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Updated: Aug 19, 2025

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

Injectable decellularized dental pulp matrix-functionalized hydrogel microspheres for endodontic regeneration.

Liwen Zheng¹, Yaxian Liu¹, Lin Jiang¹

¹Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, PR China; Stomatological Hospital of Chongqing Medical University, Chongqing 401174, PR China.

Acta Biomaterialia

|December 1, 2022

Summary

Researchers developed hydrogel microspheres with dental pulp stem cells and bioactive factors to regenerate pulp-like tissue and dentin. This approach offers a promising new treatment for dental pulp disease, improving tissue regeneration.

Keywords:

Decellularized matrix Dental pulp Endodontic regeneration Hydrogel microspheres

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Last Updated: Aug 19, 2025

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Fabricating Highly Open Porous Microspheres HOPMs via Microfluidic Technology

Published on: May 16, 2022

Novel Process for 3D Printing Decellularized Matrices

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Dental pulp disease is common, but functional pulp regeneration is difficult.
Conventional bulk biomaterials for regenerative endodontics can cause central tissue necrosis.
Hydrogel microspheres offer advantages over bulk scaffolds, but require specific bioactive factors for stem cell regulation.

Purpose of the Study:

To create hydrogel microspheres mimicking the dental pulp microenvironment using decellularized matrix-derived bioactive factors.
To evaluate the efficacy of these microspheres in promoting human dental pulp stem cell (hDPSC) differentiation and pulp-dentin regeneration.

Main Methods:

Fabrication of hydrogel microspheres incorporating decellularized dental pulp matrix-derived bioactive factors.
In vitro culture of hDPSCs on the microspheres to assess pulp-formation ability.
In vivo evaluation in a semi-orthotopic model to assess pulp-like tissue and new dentin regeneration.

Main Results:

The engineered microspheres demonstrated excellent plasticity, biocompatibility, and biological performance.
hDPSCs cultured on microspheres showed enhanced pulp-formation in vitro.
Successful regeneration of pulp-like tissue and new dentin was achieved in vivo using hDPSC-loaded microspheres.

Conclusions:

Hydrogel microspheres functionalized with dental pulp-specific bioactive factors can effectively simulate a 3D pulp microenvironment.
This strategy promotes multi-directional differentiation of hDPSCs, leading to pulp-dentin complex regeneration.
The developed microspheres represent a promising therapeutic option for dental pulp disease and regenerative endodontics.