2D Co₃O₄ modified by IrO₂ nanozyme for convenient detection of aqueous Fe²⁺ and intercellular H₂O₂

A portable sensor for visual monitoring of Fe²⁺ and H₂O₂, two-dimensional Co₃O₄ modified by nano-IrO₂ (IrO₂@2D Co₃O₄) was prepared in this work, for the first time, with the help of microwave radiation at 140 °C, which was further stabilized onto common test strips. The present IrO₂@2D Co₃O₄ possessed superior dual-function enzyme-like activity with low toxicity and excellent biocompatibility. Especially, trace Fe²⁺ and H₂O₂ could exclusively alter their enzyme-like catalytic activity with discriminating hyperchromic or hypochromic effect, i.e., from blue to colorless or to dark blue for both IrO₂@2D Co₃O₄ dispersion and its functionalized test strips. The linear regression equations were A₆₅₂ = 0.5940 - 0.00041 c_Fe²⁺ (10^-8 M, R² = 0.9927) for Fe²⁺ and ∆A₆₅₂ = 0.0023 c_H2O2 + 0.00025 (10^-7 M, R² = 0.9982) for H₂O₂, respectively. When applied to visual monitoring of aqueous Fe²⁺ and intercellular H₂O₂, the recoveries were 101.2 ~ 102.5% and 95.8 ~ 103.7% with detection limits of 1.25 × 10^-8 mol/L and 1.02 × 10^-7 mol/L, respectively, far below the permitted values in drinking water set by the World Health Organization. The mechanisms for the enhancing enzyme-mimetic activity of IrO₂@2D Co₃O₄ and its selective responses to Fe²⁺ and H₂O₂ were investigated in detail.