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Effective gossypol removal from cottonseed meal through optimized solid-state fermentation by Bacillus coagulans
Zhenting Zhang1,2, Danlu Yang1, Ling Liu1
1State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Microbial Cell Factories
|December 2, 2022
Summary
Bacillus coagulans effectively removes toxic free gossypol from cottonseed meal through solid-state fermentation. This process enhances protein content and nutritional value, making cottonseed meal a safer feed ingredient.
Area of Science:
- Agricultural Science
- Microbiology
- Biotechnology
Background:
- Cottonseed meal (CSM) is a protein-rich agricultural by-product.
- Free gossypol (FG) in CSM is toxic and limits its use in animal feed.
- Microbial fermentation is a promising method for detoxifying CSM, but efficiency varies.
Purpose of the Study:
- To evaluate Bacillus coagulans for solid-state fermentation of CSM.
- To optimize fermentation conditions for maximum FG removal.
- To assess the impact on CSM nutritional quality.
Main Methods:
- Isolated and screened 36 B. coagulans strains for FG degradation.
- Optimized solid-state fermentation parameters: temperature, moisture, inoculation, time, pH.
- Fermented CSM using B. coagulans S17 under optimal conditions.
Main Results:
- Achieved 81.83% reduction in FG content (from 923.80 to 167.90 mg/kg).
- Increased crude protein content from 47.98% to 52.82%.
- Reached high spore concentration of B. coagulans S17 (1.68 × 10^10 CFU/g).
Conclusions:
- B. coagulans demonstrates significant potential for degrading FG in CSM.
- Developed a feasible solid-state fermentation process using B. coagulans S17.
- This method improves CSM's nutritional value and resource utilization.
