Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intramolecular loops control SARS-CoV-2 nucleocapsid protein self-association and nucleic acid binding dependent on phosphorylation.

bioRxiv : the preprint server for biology·2026
Same author

Evolution of a fuzzy ribonucleoprotein complex in viral assembly.

eLife·2025
Same author

Development and characterisation of a thermoreversible sol-gel for intranasal delivery of MRI and PET imaging agents.

International journal of pharmaceutics·2025
Same author

"To be honest, no one cares": an ethnographic study of postpartum perceptions and practices after gestational diabetes in Vietnam.

BMC pregnancy and childbirth·2025
Same author

Explainable artificial intelligence for predicting rare earth elements leaching from secondary resources.

Journal of hazardous materials·2025
Same author

Evolution of a fuzzy ribonucleoprotein complex in viral assembly.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Aug 19, 2025

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

572

Isolation protocols and mitochondrial content for plasma extracellular vesicles.

Ai Nguyen1, Illarion V Turko2,3

  • 1Institute for Bioscience and Biotechnology Research, Rockville, MD, 20850, USA.

Analytical and Bioanalytical Chemistry
|December 2, 2022
PubMed
Summary

Researchers developed a new method to isolate mitochondrial extracellular vesicles (EVs). This technique effectively separates EVs from free mitochondria using immobilized heparin chromatography, crucial for studying their roles.

Keywords:
Extracellular vesiclesHeparin-SepharoseMass spectrometryMitochondriaTargeted proteomics

More Related Videos

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

2.1K
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.2K

Related Experiment Videos

Last Updated: Aug 19, 2025

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

572
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

2.1K
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
09:57

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

2.2K

Area of Science:

  • Cell Biology
  • Extracellular Vesicles Research
  • Mitochondrial Biology

Background:

  • Mitochondrial content, including extracellular vesicles (EVs) and free mitochondria, is found outside cells.
  • Mitochondrial EVs have significant physiological and pathophysiological implications.
  • Effective isolation protocols are needed to study mitochondrial EV functions.

Purpose of the Study:

  • To develop and evaluate a chromatographic isolation protocol for mitochondrial EVs.
  • To distinguish mitochondrial EVs from other mitochondrial components.
  • To assess the efficiency of different isolation steps using protein markers.

Main Methods:

  • Utilized a multiple reaction monitoring assay with isotope-labeled internal standards.
  • Quantified mitochondrial, plasma membrane, endosomal, and soluble proteins.
  • Employed ultracentrifugation, size exclusion chromatography, and immobilized heparin chromatography.
  • Normalized protein concentrations to ATP synthase alpha subunit for comparison.

Main Results:

  • Ultracentrifugation alone did not separate EVs from contaminating mitochondrial content.
  • Size exclusion chromatography was ineffective in separating EVs from non-EV mitochondrial content.
  • Immobilized heparin chromatography demonstrated high efficiency in separating mitochondrial EVs from contaminants.

Conclusions:

  • A robust protocol involving immobilized heparin chromatography is effective for isolating mitochondrial EVs.
  • This method allows for the purification of mitochondrial EVs, essential for functional studies.
  • The findings advance the understanding and isolation of extracellular mitochondrial components.