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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Related Experiment Video

Updated: Aug 19, 2025

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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A reference human induced pluripotent stem cell line for large-scale collaborative studies.

Caroline B Pantazis1, Andrian Yang2, Erika Lara1

  • 1Center for Alzheimer's and Related Dementias (CARD), National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.

Cell Stem Cell
|December 2, 2022
PubMed
Summary
This summary is machine-generated.

We identified KOLF2.1J as a high-performing human induced pluripotent stem cell (iPSC) line. This standardized iPSC line facilitates reproducible research and data integration across global stem cell studies.

Keywords:
CRISPRdifferentiationiPSCkaryotypep53pluripotentreferencesingle-cellstem cellwhole-genome

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Area of Science:

  • Stem cell biology
  • Genomics
  • Developmental biology

Background:

  • Human induced pluripotent stem cell (iPSC) lines are crucial for disease and development research.
  • Significant phenotypic variation among iPSC lines hinders data replication and integration.

Purpose of the Study:

  • To identify and validate a standardized human iPSC line for reproducible stem cell research.
  • To assess genetic stability and differentiation capacity of candidate iPSC lines.

Main Methods:

  • Whole genome sequencing and CRISPR-Cas9 gene editing for genetic characterization.
  • Phenotypic analysis including differentiation into common cell types.
  • Global head-to-head comparisons of KOLF2.1J against other iPSC lines.

Main Results:

  • KOLF2.1J demonstrated robust performance across genetic, genomic stability, and differentiation assays.
  • KOLF2.1J performed comparably or superiorly to other iPSC lines in global collaborative testing.
  • Identification of KOLF2.1J as a well-performing, standardized iPSC line.

Conclusions:

  • KOLF2.1J is a suitable candidate for a standardized human iPSC line.
  • Availability of KOLF2.1J and its derivatives promotes large-scale collaborative stem cell research.
  • Standardization of iPSC lines is essential for advancing stem cell science.