Related Experiment Video
Updated: Aug 19, 2025
In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry
Published on: April 5, 2015
Measurement of Babesia bovis infected red blood cells using flow cytometry
Pongpisid Koonyosying1, Somdet Srichairatanakool2, Saruda Tiwananthagorn3
1Graduate School of Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Abstract:
Rapid and accurate tools are needed for high-throughput in vitro antibabesial drug testing. In this study, flow cytometry for the measuring of Babesia bovis in vitro culture, was developed using SYBR Green I and compared against the results of fluorescence-based assay and microscopic assay. A high correlation of measured parasitemia was observed with high R2 value (R2 = 0.9991) between flow cytometry and microscopic analysis. The degree of antibabesial drug sensitivity against B. bovis determined by flow cytometry was 0.424 ± 0.173 μM. Similar to the results of previously published studies involving fluorescence spectrometry-based assay (0.408 ± 0.011 μM) and microscopy-based assay (0.400 ± 0.017 μM). The outcomes of this present study suggest that flow cytometry assay using SYBR Green I can potentially be useful in determining parasitemia and can serve as a rapid alternative method to antibabesial drug testing.
