Laccase encapsulation immobilized in mesoporous ZIF-8 for enhancement bisphenol A degradation

Endocrine disruptors (EDCs) such as bisphenol A (BPA) have many adverse effects on environment and human health. Laccase encapsulation immobilized in mesoporous ZIF-8 was prepared for efficient degradation of BPA. The ZIF-8 (PA) with highly ordered mesopores was synthesized using trimethylacetic acid (PA) as a template agent. On account of the improvement of skeletal stability by cross-linking agent glutaraldehyde, ZIF-8 (PA) realized laccase (FL) immobilization within the mesopores through encapsulation strategy. By replacing the template agent, the effect of pore size on the composite activity and immobilization efficiency by SEM characterization and kinetic analysis were investigated. Based on the physical protection of ZIF-8(PA) on laccase, as well as electrostatic interactions between substances and changes in surface functional groups (e.g. -OH, etc.), multifaceted enhancement including activity, stability, storability were engendered. FL@ZIF-8(PA) could maintain high activity in complex systems at pH 3-11, 10-70 °C or in organic solvent containing system, which exhibited an obvious improvement compared to free laccase and other reported immobilized laccase. Combined with TGA, FT-IR and Zeta potential analysis, the intrinsic mechanism was elaborated in detail. On this basis, FL@ZIF-8(PA) achieved efficient removal of BPA even under adverse conditions (removal rates all above 55% and up to 90.28%), and was suitable for a wide range of initial BPA concentrations. Combined with the DFT calculations on the adsorption energy and differential charge, the mesoporous could not only improve the enrichment performance of BPA on ZIFs, but also enhance the interaction stability. Finally, FL@ZIF-8(PA) was successfully applied to the degradation of BPA in coal industry wastewater. This work provides a new and ultra-high performances material for the organic pollution treatment in wastewater.