Construction of pH-responsive polydopamine coated magnetic layered hydroxide nanostructure for intracellular drug...

In recent years, using magnetic nanocomposites for controlled release of drugs and target-specific drug delivery has great potential in exploring a new method for cancer chemotherapy. Nevertheless, the low loading rate of insoluble drugs greatly restricts their efficacy and clinical application. Here, an efficient magnetic nanostructure combining Fe₃O₄ nanoparticles and layered double hydroxide (LDH) was developed and used for tumor cell inhibition. LDH was first deposited on Fe₃O₄ nanoparticles (Fe₃O₄@LDH), curcumin (Cur) was then loaded and polydopamine (PDA) eventually formed a PDA-coating on Fe₃O₄@Cur-LDH via self-polymerization. The Fe₃O₄@Cur-LDH/PDA nanostructure showed a suitable nano-meter size, excellent magnetic property, and high drug loading rate (up to 38 %). In vitro release results implied that Fe₃O₄@Cur-LDH/PDA nanostructure had good pH-responsive performance and excellent controlled-release behaviors due to the introduction of PDA. The cellular experiments demonstrated that Fe₃O₄@Cur-LDH/PDA nanostructure had good biocompatibility. In addition, Fe₃O₄@Cur-LDH/PDA entered into the cells mainly through endocytosis and had excellent inhibition on HepG2 cell viability in a concentration-dependent manner. Therefore, Fe₃O₄@Cur-LDH/PDA nanostructure has a prospective application in cancer therapy as a controlled drug delivery system.