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A new solution for initial perfusion of transplant kidneys
Scandinavian Journal of Urology and Nephrology
|January 1, 1978
Summary
Removing blood from kidneys before transplantation is crucial. A specialized perfusion solution effectively removed blood, even after warm ischemia, ensuring excellent kidney function post-transplant.
Area of Science:
- Nephrology
- Transplantation Immunology
- Organ Preservation
Background:
- Complete blood removal from donor kidneys is essential for successful transplantation.
- Warm ischemia can complicate blood removal and impact graft viability.
Purpose of the Study:
- To evaluate a novel perfusion solution for efficient blood removal from rabbit kidneys.
- To assess the impact of the perfusion solution on kidney function after transplantation, especially following warm ischemia.
Main Methods:
- Rabbit kidneys were perfused with a high Mg++ and glucose solution (400 mOsm/kg).
- Blood removal was quantified by hemoglobin assays in effluent and kidney tissue.
- Perfusion pressure was maintained at 60 mmHg.
- The effect of adding 5% dextran 40 (Rheomacrodex) was investigated.
Main Results:
- A perfusion pressure of 60 mmHg was adequate for effective blood washout.
- The specialized perfusate successfully removed blood from kidneys, even after one hour of warm ischemia.
- Adding 5% dextran 40 significantly enhanced blood removal in kidneys subjected to warm ischemia.
Conclusions:
- The developed perfusion solution effectively removes blood from donor kidneys.
- This method improves organ preservation, particularly after ischemic periods.
- Transplanted kidneys treated with this solution demonstrated excellent function.