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A Review on Spike and Recovery Method in Analytical Method Development and Validation.

Diksha Thakur¹, Neeti Prakash Dubey², Rajvinder Singh¹

¹Department of Forensic Science, M.D. University, Rohtak, Haryana, India.

Critical Reviews in Analytical Chemistry

|December 4, 2022

Summary

This summary is machine-generated.

Spike and recovery (SAR) methods are crucial for analytical testing accuracy in forensic toxicology. This review clarifies SAR

Keywords:

Forensic Toxicology Method Validation Quality Assurance Recovery Spike

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Area of Science:

Forensic Science
Analytical Chemistry
Toxicology

Background:

Analytical approaches are vital in multidisciplinary science.
Spike and recovery (SAR) is a key technique for assessing analytical method accuracy.
SAR methods are fundamental in analytical testing.

Purpose of the Study:

To clarify the role and utility of SAR methods in forensic science.
To discuss the applications and current role of SAR in Forensic Toxicology.
To highlight SAR as a critical parameter in analytical method validation.

Main Methods:

A comprehensive literature search was conducted across multiple scientific databases (Google, Web of Sciences, Scopus, PubMed, Google Scholar, SciFinder).
Scientific reports on SAR utility were critically explored from sources like Science Direct.
The review focuses on SAR applications within Forensic Toxicology.

Main Results:

SAR methodology is essential for the accurate identification and quantitation of analytes in sample matrices.
SAR is a major parameter in the validation of analytical methods.
SAR-based studies are fundamental for method development, optimization, quality control, and assurance.

Conclusions:

SAR methods are indispensable for ensuring quality assurance across scientific disciplines.
A better understanding and adherence to standard guidelines are needed for SAR utilization.
SAR plays a substantial role in analytical testing and forensic science applications.