Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

11.5K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.5K
Fluid Mosaic Model01:19

Fluid Mosaic Model

12.2K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
12.2K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.5K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.5K
Mesh Analysis01:20

Mesh Analysis

833
Mesh analysis is a valuable method for simplifying circuit analysis using mesh currents as key circuit variables. Unlike nodal analysis, which focuses on determining unknown voltages, mesh analysis applies Kirchhoff's voltage law (KVL) to find unknown currents within a circuit. This method is particularly convenient in reducing the number of simultaneous equations that need to be solved.
A fundamental concept in mesh analysis is the definition of meshes and mesh currents. A mesh is a closed...
833
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

5.1K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
5.1K
What are Membranes?01:54

What are Membranes?

158.2K
A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and...
158.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid headgroup composition and pH modulate the cholesterol requirement for pneumolysin activity.

International journal of biological macromolecules·2026
Same author

The temperature dependence of amyloid <i>β</i> solubility reveals the hydrophobic effect as the main driving force for fibril formation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Transient Interactions of α-Synuclein N- and C-Termini.

ACS chemical neuroscience·2026
Same author

Effect of pH on niacinamide skin permeation.

Scientific reports·2026
Same author

Exploring MPM-FLIM for diagnostics of porokeratosis - a pilot study <i>ex vivo</i>.

Biomedical optics express·2026
Same author

Structural defects in amyloid-β fibrils drive secondary nucleation.

Nature communications·2026

Related Experiment Video

Updated: Aug 19, 2025

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.4K

Membrane permeability based on mesh analysis.

Björn Stenqvist1, Marica B Ericson2, Sebastien Gregoire3

  • 1Division of Physical Chemistry, Department of Chemistry, Lund University, POB 124, SE-221 00 Lund, Sweden.

Journal of Colloid and Interface Science
|December 4, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a new Mesh analysis method for modeling composite membranes, accurately predicting diffusion and validating findings against skin stratum corneum experiments. The method reveals significant impacts of lipid fluidity and arrangement on membrane permeability.

Keywords:
Brick and mortarFick’s lawMembraneStratum corneumTortuosity

More Related Videos

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.4K

Related Experiment Videos

Last Updated: Aug 19, 2025

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.4K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.4K

Area of Science:

  • Biophysics
  • Materials Science
  • Computational Modeling

Background:

  • Membranes control matter exchange, crucial for biological and technical systems.
  • Composite membranes exhibit spatially varying transport properties.
  • Accurate modeling of composite membranes is essential for understanding their function.

Purpose of the Study:

  • To develop an asymptotically exact method for modeling diffusion in composite membranes.
  • To investigate the influence of lipid properties and structure on membrane permeability.
  • To provide a computational approach for analyzing skin barrier function.

Main Methods:

  • Reformulated generalized Fick's law with a condensed parameter.
  • Applied Mesh analysis, akin to a finite element method, for diffusion modeling.
  • Validated the model using experimental data for stratum corneum.

Main Results:

  • The developed method accurately predicts concentration profiles in composite membranes.
  • Significant impact of lipid fluidity gradients and anisotropic lipid organization on permeability was observed.
  • Corneocyte swelling and lateral arrangement were shown to affect overall membrane permeability.

Conclusions:

  • The Mesh analysis method offers an accurate approach for composite membrane diffusion modeling.
  • Lipid properties and structural organization within the stratum corneum significantly influence its barrier function.
  • This model provides insights into skin permeability and can be applied to other composite membrane systems.