Related Experiment Video
Updated: Aug 19, 2025
Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels
Published on: February 14, 2018
The functional link between TCTP and Drsl1 in deltamethrin-stressed Drosophila Kc cells
Man Zhang1, Xiaoli Ying1, Luogen Cheng1
1Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Abstract:
Translationally controlled tumor protein (TCTP) is a growth and development related protein found in almost all eukaryotes and can be involved in a variety of cellular life activities. Our previous studies found that TCTP is involved in the response of Drosophila Kc cells to deltamethrin (DM) stimulation, and it may be a candidate gene related to DM stress. Therefore, we would further investigate the functions of TCTP and its mechanism under DM stress. The qPCR results showed that the expression level of Drsl1 increasing first and then decreasing with the change of DM concentration and treatment duration. The optimal concentration was 20 ppm and the optimal time was 24 h. qPCR and WB results together showed that the expression levels of TCTP and Drsl1 were positively correlated. The flow cytometry showed the expression levels of TCTP and Drsl1 in deltamethrin-stressed cells are related to apoptosis. The apoptosis rate reached the highest level in the cells with simultaneous interference of both genes. Taken together, our data mainly suggest that TCTP interacts with Drsl1 in response to DM stress in Drosophila Kc cells, which helps to investigate the mechanisms of DM toxicity and the mechanisms by which insects develop resistance to it.
Insights
Translationally controlled tumor protein (TCTP) interacts with Drsl1 in Drosophila Kc cells under deltamethrin (DM) stress. This interaction influences DM toxicity and insect resistance mechanisms, impacting cellular apoptosis.
Area of Science:
- Cell Biology
- Molecular Toxicology
- Insect Physiology
Background:
- Translationally controlled tumor protein (TCTP) is a conserved eukaryotic protein involved in cellular functions.
- Previous research suggests TCTP plays a role in Drosophila Kc cell responses to deltamethrin (DM) stress.
- Further investigation is needed to elucidate TCTP's function and mechanism under DM stress.
Purpose of the Study:
- To investigate the function of TCTP and its mechanism in Drosophila Kc cells under deltamethrin (DM) stress.
- To explore the relationship between TCTP and Drsl1 expression in response to DM.
- To determine the role of TCTP and Drsl1 in DM-induced apoptosis.
Main Methods:
- Quantitative real-time PCR (qPCR) to analyze gene expression levels.
- Western blotting (WB) to assess protein expression.
- Flow cytometry to evaluate cellular apoptosis rates.
Main Results:
- Deltamethrin (DM) concentration and duration affect Drsl1 expression, with optimal conditions at 20 ppm for 24 hours.
- Expression levels of TCTP and Drsl1 were found to be positively correlated.
- Simultaneous interference of TCTP and Drsl1 led to the highest apoptosis rate in DM-stressed cells.
Conclusions:
- TCTP interacts with Drsl1 in Drosophila Kc cells during deltamethrin (DM) stress.
- This interaction is linked to cellular apoptosis and may contribute to understanding DM toxicity.
- The findings provide insights into insect resistance mechanisms against deltamethrin.
