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The E2F6 Transcription Factor is Associated with the Mammalian SUZ12-Containing Polycomb Complex
Yuko Shirahama1, Ken Yamamoto1
1Department of Medical Biochemistry, Kurume University School of Medicine.
The Kurume Medical Journal
|December 4, 2022
Summary
Polycomb group (PcG) protein SUZ12 interacts with repressor E2F6, forming a complex with EZH2. This complex regulates gene expression, suggesting a novel gene activation mechanism by PcGs.
Area of Science:
- Epigenetics
- Molecular Biology
- Gene Regulation
Background:
- Polycomb group (PcG) proteins, including SUZ12 and EZH2, are crucial for epigenetic maintenance of gene silencing.
- The recruitment mechanisms of PcG complexes to target genes remain poorly understood.
Purpose of the Study:
- To investigate the interaction between SUZ12 and E2F family proteins.
- To elucidate the role of the SUZ12-EZH2 complex in gene regulation.
Main Methods:
- Biochemical purification of the SUZ12-EZH2-E2F6 complex from HEK293 cells.
- Chromatin immunoprecipitation (ChIP) assays to identify target genes.
Main Results:
- SUZ12 selectively interacts with the transcriptional repressor E2F6, forming a complex with EZH2.
- The E2F6-SUZ12-EZH2 complex targets specific genes, whose promoters show low H3K27 trimethylation.
- Depletion of EZH2 leads to down-regulation of these target genes' expression.
Conclusions:
- The E2F6-SUZ12-EZH2 complex is recruited to target genes.
- The interaction inhibits E2F6-mediated repression, suggesting a role in gene activation by PcGs, contrary to their typical repressive function.
Keywords:
E2F transcription factorchromatin immunoprecipitationhistone methylationpolycomb group proteintranscriptional regulationMore Related Videos
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