Related Experiment Video

Updated: Aug 19, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:39

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

223

Establishment of a risk model correlated with metabolism based on RNA-binding proteins associated with cell

Ting Bin1, Chao Lin2, Fang-Jie Liu1

  • 1Department of Haematology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.

Frontiers in Oncology
|December 5, 2022
PubMed
Abstract

Insights

This study identifies key RNA-binding proteins (RBPs) involved in pyroptosis to predict acute myeloid leukemia (AML) prognosis. A novel risk model using these genes accurately forecasts patient survival and highlights metabolic and immune pathway roles.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • RNA-binding proteins (RBPs) regulate gene expression in acute myeloid leukemia (AML).
  • Pyroptosis, a programmed cell death pathway, modulates the immune response in AML.
  • Identifying prognostic biomarkers in AML is crucial for patient management.

Purpose of the Study:

  • To identify pyroptosis-related RNA-binding protein (RBP) genes.
  • To develop a predictive model for acute myeloid leukemia (AML) patient prognosis.
  • To explore the functional pathways associated with different risk groups in AML.

Main Methods:

  • Downloaded AML expression data from public databases (UCSC, GEO).
  • Constructed a protein-protein interaction (PPI) network to identify key pyroptosis-RBP differentially expressed genes (PRBP-DEGs).
  • Developed a prognostic risk model using Cox analysis and validated it with ROC and survival curves, including nomogram construction and GSEA.

Main Results:

  • Identified 71 PRBP-DEGs and established a risk model using IFIT5, MRPL14, MRPL21, MRPL39, MVP, and PUSL1.
  • RiskScore, age, and cytogenetics were independent prognostic factors, with the nomogram accurately predicting 1-, 3-, and 5-year survival.
  • GSEA revealed enrichment in metabolic and immune-related processes for high- and low-risk groups.

Conclusions:

  • Established a novel risk score model based on pyroptosis-related RBPs for AML prognosis.
  • The model, incorporating metabolic correlations, offers a valuable reference for AML therapeutic strategies and outcome prediction.
  • This research provides a foundation for understanding RBP roles in AML pathogenesis and immune regulation.
Keywords:
RNA-binding proteinacute myeloid leukemiametabolismprognostic modelpyroptosis

More Related Videos

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype14:51

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

3.3K
Assessment of the Metabolic Profile of Primary Leukemia Cells06:21

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

10.6K

Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.8K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.7K