Related Experiment Video

Updated: Aug 19, 2025

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
10:22

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

9.0K

Plasmodium falciparum Malaria Susceptibility and Severity: Influence of MyD88-Adaptor-Like Gene (rs8177374)

Amany I Ammar1, Sally M El-Hefnawy2, Somaia Shehab-Eldeen3,4

  • 1Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin Elkom, Egypt.

Infection and Drug Resistance
|December 5, 2022
PubMed
Abstract

Insights

Single nucleotide polymorphism (SNP) in the MyD88-adapter-like (MAL) gene, specifically rs8177374, influences malaria risk. The mutant MAL rs8177374 variant may offer protection against severe Plasmodium falciparum malaria.

Area of Science:

  • Immunogenetics
  • Infectious Diseases
  • Molecular Biology

Background:

  • MyD88-adapter-like (MAL) is a crucial adapter protein for Toll-like receptors (TLRs), modulating inflammatory responses.
  • Single nucleotide polymorphisms (SNPs) in MAL can affect its function and influence susceptibility to infectious diseases.
  • The study investigates the impact of the MAL rs8177374 polymorphism on Plasmodium falciparum malaria.

Purpose of the Study:

  • To examine the association between the MAL rs8177374 polymorphism and susceptibility to Plasmodium falciparum malaria.
  • To assess the influence of the MAL rs8177374 polymorphism on the severity of Plasmodium falciparum malaria.
  • To evaluate the relationship between MAL rs8177374 genotypes and levels of TNF-alpha and IL-12.

Main Methods:

  • Case-control study involving 141 malaria patients and 147 healthy controls.
  • Genotyping of the MAL rs8177374 polymorphism using allele-specific PCR.
  • Measurement of TNF-alpha and IL-12 levels via ELISA.

Main Results:

  • The MAL rs8177374 (CT) genotype was linked to increased malaria risk (OR: 2.52).
  • CT and TT genotypes provided significant protection against severe malaria (ORs < 0.19).
  • The T allele increased malaria risk (OR: 1.7) but protected against severe malaria (OR: 0.135). Mutant genotypes showed higher TNF-alpha and IL-12 levels.

Conclusions:

  • Single nucleotide polymorphism in the MyD88-adaptor-like gene is associated with malaria risk.
  • Individuals with the MAL rs8177374 mutant variant may have reduced susceptibility to severe malaria.
  • The rs8177374 polymorphism influences inflammatory cytokine levels, potentially affecting malaria outcomes.
Keywords:
IL-12MALP. falciparumTNF-alphamalaria

More Related Videos

A Multi-detection Assay for Malaria Transmitting Mosquitoes09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

13.3K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.8K

Related Concept Videos

Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.7K
Multiple Allele Traits01:49

Multiple Allele Traits

The Concept of Multiple Allelism
34.5K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.6K