Differences among reciprocal hybrids of Labeotropheus

Current evidence suggests that hybridization played a crucial role in the early evolution and diversification of the species flocks of cichlid fishes in the African Great Lakes. Nonetheless, evidence for hybridization in the extant cichlid fauna is scant, suggesting that hybridization is rare in the modern era, perhaps enforced by natural or sexual selection acting against F1 hybrids. Additionally, most experimental studies of hybridization perform a hybrid cross in one direction, ignoring the reciprocal hybrid. In this study, we perform reciprocal crosses between sympatric congeners from Lake Malaŵi, Labeotropheus fuelleborni and L. trewavasae, in order to compare the body shape and coloration of males of both of these hybrids, as well as to examine how these hybrids fare during both inter- and intrasexual interactions. We found that L. trewavasae-sired hybrid males are intermediate to the parental species both morphologically and chromatically, while the reciprocal L. fuelleborni-sired hybrids are likely transgressive hybrids. Males of these transgressive hybrids also fare poorly during our mate choice experiments. While female L. trewavasae reject them as possible mates, male L. trewavasae do not make a distinction between them and conspecific males. Selection against transgressive F1 hybrids as observed in our crossing experiments may help explain why contemporary hybridization in Lake Malaŵi cichlids appears to be rare.

The online version contains supplementary material available at 10.1007/s10750-022-05092-4.