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Related Experiment Video

Updated: Aug 18, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

p53 immunostaining pattern is a useful surrogate marker for TP53 gene mutations.

You-Na Sung¹, Deokhoon Kim¹, Jihun Kim²

¹Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-Ro 43-Gil, Songpa-Gu, Seoul, 05505, Korea.

Diagnostic Pathology

|December 5, 2022

Summary

p53 immunohistochemical staining (IHC) patterns effectively predict TP53 gene mutations in solid cancers. MDM2 amplification status does not influence p53 IHC patterns, aiding cancer diagnosis and treatment.

Keywords:

Association Immunohistochemistry Mutation TP53 gene p53 protein

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

TP53 is the most frequently mutated gene in human cancers.
Understanding TP53 mutational status is crucial for cancer patient diagnosis and treatment.
MDM2 acts as a negative regulator of p53.

Purpose of the Study:

To investigate the association between TP53 gene mutations and p53 immunohistochemical staining (IHC) patterns.
To evaluate the non-genetic effect of MDM2 on p53 IHC patterns.

Main Methods:

135 solid cancer cases with next-generation sequencing data were analyzed.
p53 IHC was performed and patterns (overexpression, null type, usual) were classified.
TP53 mutations were annotated into DISRUPTED and IF-DBD categories.

Main Results:

TP53 mutations were observed in 77.0% of cases.
Null type p53 IHC pattern correlated with DISRUPTED TP53 mutations (86.2% sensitivity, 97.2% specificity).
Overexpression p53 IHC pattern correlated with IF-DBD TP53 mutations (100% sensitivity, 81.7% specificity).
Usual p53 IHC pattern showed 100% specificity for predicting wild-type TP53.
p53 IHC patterns showed a perfect association with TP53 mutation patterns, irrespective of MDM2 amplification.

Conclusions:

p53 IHC patterns (overexpression, null type, usual) are reliable predictors of TP53 mutational status (DISRUPTED, IF-DBD).
MDM2 amplification status does not impact p53 IHC patterns.