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Updated: Aug 18, 2025

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
Published on: May 3, 2013
A 3D view of how enteroviruses hijack autophagy
Selma Dahmane1,2,3,4, Kasturika Shankar1,2,3,4, Lars-Anders Carlson1,2,3,4
1Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
Abstract:
Viruses are masters at using cellular pathways to aid their replication. Cryo-electron tomography of poliovirus-infected cells revealed how it utilizes macroautophagy to its advantage. Assembly of these non-enveloped virions takes place directly on membranes and requires PIK3C3/VPS34 activity to be completed, whereas the canonical autophagy inducer ULK1 restricts virus assembly. The tomograms further revealed that enterovirus-induced autophagy is selective for RNA-loaded virions, which may help ensure maximum infectivity of the virus-laden vesicles released through secretory autophagy.
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