Insect phenoloxidase and its diverse roles: melanogenesis and beyond

Insect life on earth is greatly diversified despite being exposed to several infectious agents due to their diverse habitats and ecological niche. One of the major factors responsible for their successful establishment is having a powerful innate immune system. The most common and effective method used by insects in recognizing pathogen and non-self-substances is the melanization process among others. The key enzyme involved in melanin biosynthesis is the copper containing humoral defense enzyme, phenoloxidase (PO). This review focused on understanding about PO and that had been in research for nearly a century. The review elaborates about evolutionary significance of PO in arthropods, its relationship with mammalian tyrosinases, various substrates, activators and inhibitors involved in the activation of phenoloxidase cascade, as it requires an integrated system of activation that vary among insect species. The enzyme also plays a vital role in insect immunity by involving in several other immune functions like sclerotization, wound healing, opsonization, encapsulation and nodule formation. Further, gene knock down or knock out of PO genes and inhibition of PO-melanization cascade by several mechanisms can also be considered as promising future alternative to control serious pests by making them highly susceptible to any targeted attack.