Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met)
Sandra Horschitz1, Josef Frank2, Philipp Koch1
1Hector Institute for Translational Brain Research, Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, Germany; German Cancer Research Center, Heidelberg, Germany.
Abstract:
Brain-derived neurotrophic factor (BDNF) has been implicated in a multitude of neurodevelopmental processes including neuronal differentiation, axonal outgrowth, synaptic plasticity, or survival. One human-specific single nucleotide polymorphism (rs6265) in the BDNF gene causes a substitution of valine (Val) to methionine (Met) at codon 66 in the pro domain of the protein (Val66Met). This substitution is associated to reduced hippocampal volumes, poor performance on hippocampal-dependent memory tasks, and some mental disorders such as schizophrenia, depression or Alzheimer's disease. Here we generated three iPSC lines from healthy donors, either homozygous (Val/Val and Met/Met) or heterozygous (Val/Met) for the polymorphism.
More Related Videos
07:33Conversion of Human Induced Pluripotent Stem Cells iPSCs into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
Published on: May 1, 2019
09:32Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
Published on: November 11, 2015
