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Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease

Yoshinao Muro1, Yuta Yamashita1, Haruka Koizumi1

  • 1Department of Dermatology, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.

Journal of Immunological Methods
|December 6, 2022
PubMed
Abstract

No abstract available in PubMed .

Keywords:
Anti-centromere antibodyEpitopeImmunoassaySjögren's syndromeSystemic

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.