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Related Concept Videos

CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
131
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Homologous Recombination02:31

Homologous Recombination

50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.1K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.1K

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Related Experiment Video

Updated: Aug 18, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.9K

[Genome editing technology: from basics to applications].

Minori Tamai1

  • 1The Department of Pediatrics, University of Yamanashi.

[Rinsho Ketsueki] the Japanese Journal of Clinical Hematology
|December 8, 2022
PubMed
Summary

The CRISPR/Cas9 system, a bacterial immune mechanism, is a powerful genome editing tool for mammalian cells. It enables gene knockout and precise DNA modifications for research and clinical use.

Area of Science:

  • Molecular Biology
  • Genetics

Context:

  • CRISPR/Cas9 system originated from bacterial adaptive immunity.
  • It has been adapted for genome editing in eukaryotic cells, particularly mammalian systems.

Purpose:

  • To explain the fundamental principles of the CRISPR/Cas9 system.
  • To discuss its applications in gene editing, including gene knockout and sequence insertion.
  • To highlight advanced uses such as transcriptional regulation and epigenetic modification.

Summary:

  • The CRISPR/Cas9 system comprises crRNA, tracrRNA, and Cas9 protein to induce targeted DNA double-strand breaks.
  • DNA repair mechanisms (NHEJ or HDR) facilitate gene knockout or sequence insertion.
  • The technology's simplicity has led to widespread adoption in research and clinical settings.
Keywords:
CRISPR/Cas9Genome editingdCas9nCas9

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

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Related Experiment Videos

Last Updated: Aug 18, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Impact:

  • Facilitates gene knockout and precise nucleotide substitutions for downstream analyses.
  • Enables diverse applications including transcriptional regulation, epigenetic modification, and live-cell imaging.
  • Considerations for effective genome editing include minimizing off-target effects and optimizing delivery methods.